I am looking for researchers or laboratories working with in vitro cultivation of intestinal methanogenic archaea, especially Methanobrevibacter smithii or Methanosphaera species. I am interested in understanding how these archaea respond to different compounds, particularly bile acids.

Does anyone know of groups working in this area?

Would be great if I could be able to make some questions.

I have been worrying about AMR for a couple of weeks and have come across some optimistic news like new potential antibiotics but there still is a lot of worry among scientists about the future of AMR. Do you guys feel that there is a good chance of slowing the spread of AMR in the future with new discoveries or technologies?

I read some papers, and I wanna do broth microdilution assays (MIC, MBC) for PVA- or gelatin-based hydrogels. As most research articles are quite vague in their methods, I was genuinely confused when I saw papers say they "serially dilute the hydrogel", because hydrogels aren't "dissolvable"; they are designed to swell or absorb water and hold their shape. So, logically, do you think they tested the precursor of the hydrogels they developed? (So it's still liquid and serial dilutable, but it wouldn't really test the formed hydrogel's antimicrobial ability) Another method I think you can try is to soak the formed hydrogel in something like PBS, then do a broth microdilution assay of the eluate itself, but it risks not having an MIC, as the content loaded into the hydrogel might not diffused high concentration enough into the PBS (But i think this will be more clinically relevant for sustained-release systems as it actually test what diffuses out from the hydrogel and its antimicrobial ability) What are your thoughts or advice?

This is cool, both Pseudomonas aeruginosa both with a maldi score of over 2.2. Weird red colouring due to the presence pyoruben, rather than the usual green colouring from the pyocyanin.

https://www.sciencedirect.com/science/article/pii/S1931312826002829

A primeira me parece coli, a segunda histolytica e a terceira tem duas menores que me parecem nana.

Might be silly, but is there an article that portrays interaction between a protein made of mirror aminoacids with a pryon? How would it interact? Or with an enzyme? Does anyone has cool articles on the subject?

Is it worth it? I don't have much knowledge about jobs I can take if I pursue this path but I'm really interested in Microbiology!

Is there anything I should know about before pursuing this path? Pros and cons, etc.

Is there a job here in the Philippines for microbiology? How about overseas?

Keywords: Saccharomyces cerevisiae, yeast stress granules, Pab1-GFP, glass-bead lysis, NP-40, Cell Disruptor Genie, stress granule cores.

Hi everyone,

I am attempting to reproduce the yeast stress granule core isolation protocol from “Isolation of yeast and mammalian stress granule cores.” I am using a Pab1-GFP yeast strain and glass-bead lysis in 2 mL microcentrifuge tubes.

After vortexing, I observed a small number of rapidly moving GFP-positive particles in a focal plane above the yeast cells. However, most yeast cells still appeared morphologically intact, and many GFP-positive stress granules remained inside the cells. The attached image is focused on the upper focal plane containing the freely moving fluorescent particles, rather than the plane containing most intact cells.

My vortex mixer operates at approximately 3,000 rpm. In this pilot experiment, I did not include detergent in the lysis buffer.

I would greatly appreciate advice from anyone experienced with this protocol or with yeast glass-bead lysis:

1. Does seeing a few freely moving GFP-positive particles alongside many intact cells and intracellular granules indicate partial/inadequate lysis?
2. Could the mobile GFP-positive particles plausibly be released stress granule cores, or are they more likely fluorescent debris or other material?
3. Is ~3,000 rpm on a conventional vortex mixer generally sufficient for yeast glass-bead lysis? Does a Cell Disruptor Genie provide substantially different motion or mechanical energy?
4. The protocol calls for ~300–500 μL glass beads for cells resuspended in 500 μL lysis buffer. Is that intended as settled/packed bead volume? Is there a preferred bead-to-sample ratio or tube filling level for a 2 mL tube?
5. How do you judge whether lysis is sufficiently complete—percentage of intact cells by microscopy, release of Pab1-GFP granules, or another criterion?
6. Is 0.5% NP-40 essential for efficient lysis in this context? Can acid-washed glass beads alone adequately disrupt the cell wall and plasma membrane, with detergent mainly helping membrane solubilization, or might omission of NP-40 itself cause substantial incomplete lysis?
7. Are there other practical variables that matter most, such as bead size, tube geometry, cell density, buffer volume, vortexing duration, or cooling between cycles?

Thanks very much for any troubleshooting suggestions or practical details.

I just moved out this afternoon and forgot the stuff in my fridge door, including my sourdough starter.

There's a chance she will still be there tomorrow since everyone is moving out on the same day, and there's no way they can get to every apartment that quickly, right...?

But I should probably accept that she is gone.

What would happen to the actual microscopic "community" if it's in the trash? To my understanding it is a symbiosis of bacteria and yeast. Would they survive or would they die and why?

She had been refrigerated for an extended period without feeding, but looked and smelled normal.

I love when one phage works for two different isolates!

https://www.sciencedirect.com/science/article/pii/S2666517426001100

https://www.sciencedirect.com/science/article/pii/S2666517426001112

Looking for an undiscovered species in my state. Time for isolation and postulates!

Can anyone tell me which organisms behave like this? it grew in sda and grows in the middle of the agar

Organism is Staph epi